Balasubramaniam NAMASIVAYAM : "Determination of the performance of the NanoBioAnalytique platform for detection and the characterization of extracellular vesicles: exploration of human platelet lysates for neuroprotective"

Abstract : 

Extracellular vesicles (EVs) are heterogeneous vesicular structures ranging in size from 50 to 5000 nm. They are released by all cell types and difficult to distinguish due to their heterogeneity, the overlap of their physical properties with other components of complex media such as protein aggregates, lipoproteins, etc. NBA) was developed as a multiplexed analytical solution for efficient, non-labeling characterization of EVs. It is a multi-bio-physical characterization technique composed of surface plasmon resonance imaging (SPRi), atomic force microscopy (AFM) and

mass spectrometry (MS). The first axis of this work consisted in evaluating the analytical performance of the NBA platform for a complex biological sample of VE, derived from platelets (PVE) provided by Taipei Medical University (TMU). A biochip specific multiplex format was designed using antibodies against the CD41 protein commonly expressed in PVEs. EVs are immunocaptured by an antibody biochip grafted at two different concentrations and over a concentration range spanning 4 decades, followed by in situ nanocharacterization by AFM in order to study their structural and morphological characteristics. The quantitative analysis of EVs is also carried out by counting EVs, as well as by a fine metrological analysis (...)

Jury Composition :

M. Wilfrid BOIREAU, Directeur de recherche, Université Bourgogne Franche-Comté - PhD Director
Mme Céline ELIECAILLE, Maître de conférences, Université Bourgogne Franche-Comté - PhD Co-director
M. Luc BUEE, Directeur de recherche, Université Lille, Inserm - Reviewer
M. Jérôme DEJEU, Maître de conférences Universite Grenoble Alpes -  Reporter
M. Rienk NIEUWLAND, Assistant professor Amsterdam UMC -  Reporter
M. Thierry BURNOUF, Professeur Taipei Medical University- PhDCo-Director

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